Grace Louie

Project

Impact of Biogenic Amine Ingestion on Tissue-Specific Endogenous Levels in Anopheles stephensi Mosquitoes

Malaria is one of the most prevalent diseases worldwide, with nearly half of the world residing in regions at risk of transmission. Adult patients with severe malaria have altered concentrations of serotonin and histamine in their blood compared to healthy individuals. Previous work showed the ingestion of serotonin and/or histamine concentrations associated with adult patients with severe malaria influenced key mosquito behaviors, such as the tendency to take a second blood meal, flight behavior, and visual object inspection—traits that are related to the transmission of malaria. However, the mechanisms by which serotonin and histamine modulate mosquito behavior remain unclear. By comparing endogenous versus exogenous biogenic amine levels in An. stephensi, we hope to understand the modulation of biogenic amine distribution in An. stephensi tissue and offer insights into possible connections between neuromodulators and behavior in the mosquitoes.

What I learned

Through my research, I have not only gained technical skills and applied coursework concepts to real-world experiments but also grown personally and professionally. Entering the Riffell Lab with no background in neuroecology or sensory biology, I developed a deep appreciation for the field while learning to be more confident and independent in my work. I also came to understand how deeply collaborative research is and how much it depends on communication, diverse perspectives, and mutual support. Above all, I am grateful to the Riffell Lab for showing me the importance of creating an environment that encourages curiosity and guiding me as I continue to grow as a researcher.

Advice for future applicants

My biggest piece of advice is to take time to reflect on what your research truly means to you. It’s easy to get caught up in the details, the data, the deadlines, the uncertainty, but I believe passion and authenticity are what truly carry your work forward. Every project has meaning, and it’s important to remind yourself why you started and who you’re doing it for.

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