Mia Grayson

Project

Discovery of novel genes involved in methanotroph growth on low methane by genome comparison

Methane is a potent greenhouse gas, with a warming potential 86 times greater than that of CO2 on a 20 year time frame. Lidstrom Lab explores mitigation techniques utilizing the natural metabolism of methanotrophs, or methane-oxidizing bacteria. In my project, I compare the genomes of two closely related methanotrophs–Methylotuvimicrobium buryatense (strain 5GB1C) and Methylotuvimicrobium alcaliphilum (strain 20Z)–to identify unknown genes potentially involved in the ability to effectively use low methane concentrations, an essential property in practical methane bioremoval. I use this information to perform targeted mutations and test for changes in growth under low methane conditions. By increasing our knowledge of methanotroph genomes and optimizing single carbon bacterial metabolism, we aim to divert methane emissions and lessen global warming, while simultaneously producing valuable byproducts such as biomass feedstocks.

What I learned

Apart from the specifics of methanotrophs and laboratory microbiology, I have learned to trust my own skills in the lab to find creative solutions and direct my own work. I also have incredible mentors, and I have learned to ask for help fearlessly when I am truly unsure.

Advice for future applicants

Setbacks and surprises are the rule, not the exception. Don’t be discouraged when things don’t go as expected; you are learning just as much when you are forced to think around unanticipated problems and obstacles in your work.

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